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recombinant human tp53 protein  (TargetMol)


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    TargetMol recombinant human tp53 protein
    Recombinant Human Tp53 Protein, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human tp53 protein/product/TargetMol
    Average 94 stars, based on 16 article reviews
    recombinant human tp53 protein - by Bioz Stars, 2026-03
    94/100 stars

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    AHNAK mediates the EMT to promote PDAC cell proliferation and migration via <t>TP53</t> signaling pathway. A: GSEA was carried out in the paired PDAC samples in GEO database. It showed that most biomarkers were involved in the EMT phenotype. B: Western blotting analysis showed that AHNAK suppression caused an increase in E-cadherin but a decrease in N-cadherin. C and D: The qPCR results showed the alteration of gene expression in EMT phenotype after knockdown of AHNAK.
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    AHNAK mediates the EMT to promote PDAC cell proliferation and migration via <t>TP53</t> signaling pathway. A: GSEA was carried out in the paired PDAC samples in GEO database. It showed that most biomarkers were involved in the EMT phenotype. B: Western blotting analysis showed that AHNAK suppression caused an increase in E-cadherin but a decrease in N-cadherin. C and D: The qPCR results showed the alteration of gene expression in EMT phenotype after knockdown of AHNAK.
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    AHNAK mediates the EMT to promote PDAC cell proliferation and migration via TP53 signaling pathway. A: GSEA was carried out in the paired PDAC samples in GEO database. It showed that most biomarkers were involved in the EMT phenotype. B: Western blotting analysis showed that AHNAK suppression caused an increase in E-cadherin but a decrease in N-cadherin. C and D: The qPCR results showed the alteration of gene expression in EMT phenotype after knockdown of AHNAK.

    Journal: Journal of Cancer

    Article Title: Upregulation of nucleoprotein AHNAK is associated with poor outcome of pancreatic ductal adenocarcinoma prognosis via mediating epithelial-mesenchymal transition

    doi: 10.7150/jca.31291

    Figure Lengend Snippet: AHNAK mediates the EMT to promote PDAC cell proliferation and migration via TP53 signaling pathway. A: GSEA was carried out in the paired PDAC samples in GEO database. It showed that most biomarkers were involved in the EMT phenotype. B: Western blotting analysis showed that AHNAK suppression caused an increase in E-cadherin but a decrease in N-cadherin. C and D: The qPCR results showed the alteration of gene expression in EMT phenotype after knockdown of AHNAK.

    Article Snippet: Recombinant p53 protein was purchased from Origene (Catalog: TP710022) and Cell culture experiments were used to verify the predictions.

    Techniques: Migration, Western Blot, Expressing

    AHNAK mediates the EMT via TP53 signaling pathway. A: Western blot analysis showed that after knockdown of AHNAK, only expression of TP53 was altered, while other markers, including AKT and ERK. The phosphorylated level of TP53, AKT and ERK also showed no alterations. B: qRT-PCR showed the alteration of markers in TP53 signaling pathway. C: GSEA suggested an enrichment of TP53 signaling pathway in PDAC by analyzing the GEO database. D: Western blot showed after the recombinant TP53 protein was added into the AHNAK deficiency cell lines, E-cad, the epithelial markers of EMT phenotype was restored. E and F: CCK-8 and Transwell assays showed that recombinant TP53 could restore cellular proliferation and migration.

    Journal: Journal of Cancer

    Article Title: Upregulation of nucleoprotein AHNAK is associated with poor outcome of pancreatic ductal adenocarcinoma prognosis via mediating epithelial-mesenchymal transition

    doi: 10.7150/jca.31291

    Figure Lengend Snippet: AHNAK mediates the EMT via TP53 signaling pathway. A: Western blot analysis showed that after knockdown of AHNAK, only expression of TP53 was altered, while other markers, including AKT and ERK. The phosphorylated level of TP53, AKT and ERK also showed no alterations. B: qRT-PCR showed the alteration of markers in TP53 signaling pathway. C: GSEA suggested an enrichment of TP53 signaling pathway in PDAC by analyzing the GEO database. D: Western blot showed after the recombinant TP53 protein was added into the AHNAK deficiency cell lines, E-cad, the epithelial markers of EMT phenotype was restored. E and F: CCK-8 and Transwell assays showed that recombinant TP53 could restore cellular proliferation and migration.

    Article Snippet: Recombinant p53 protein was purchased from Origene (Catalog: TP710022) and Cell culture experiments were used to verify the predictions.

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Recombinant, CCK-8 Assay, Migration